PetriKey

Bacterium

Lactobacillus species

Doderlein vaginal-flora acid producer

lak-toh-buh-SIL-us

Gram positivegram-positiverodcatalase-negativevaginal-floralactic-acid

High-yield clue

Dominant healthy vaginal flora that produces lactic acid (and hydrogen peroxide) to keep a low pH; their loss allows bacterial vaginosis.

Overview

Gram-positive, catalase-negative rods (Doderlein bacilli) that dominate the healthy vaginal microbiota and are minor members of normal gut and oral flora. They are the classic teaching example of protective acid-producing normal flora.

Classification

  • Gram-positive
  • Rod
  • Facultative/aerotolerant anaerobe
  • Catalase-negative
  • Lactic-acid producer

Lab & identification clues

  • Gram-positive rods (Doderlein bacilli)
  • Catalase-negative
  • Lactic-acid fermentation
  • Non-spore-forming

Associations

  • Normal vaginal and gut flora
  • Protective acidification of the vagina
  • Fermentation / probiotic study framing

Commonly confused with

  • Gardnerella vaginalis
  • Listeria monocytogenes

Your notes

Original student-study summary. Sources checked: OpenStax Microbiology 2e, NCBI Bookshelf Medical Microbiology, and CDC topic pages where applicable; reviewed 2026-06. Educational only; no diagnosis, treatment, dosing, or specimen-handling guidance.

OpenStax: Microbiology 2e organism classification foundationssourceNCBI Bookshelf: Medical Microbiology organism chapterssourceCDC: CDC disease and public-health topic pagessource