PetriKey

Bacterium

Gardnerella vaginalis

Clue-cell bacterial vaginosis rod

gard-nuh-REL-uh vaj-ih-NAH-lis

gram-variablepleomorphicanaerobevaginal-floraclue-cells

High-yield clue

Clue cells (vaginal epithelial cells coated with bacteria) plus a positive amine 'whiff' test and elevated vaginal pH are the classic bacterial vaginosis clues.

Overview

A pleomorphic, gram-variable facultative anaerobe that is the main marker organism of bacterial vaginosis, a polymicrobial overgrowth. It is the standard teaching example of 'clue cells'.

Classification

  • Gram-variable
  • Pleomorphic rod/coccobacillus
  • Facultative anaerobe
  • Non-motile

Lab & identification clues

  • Clue cells on wet mount
  • Gram-variable pleomorphic staining
  • Positive amine (whiff) test with KOH
  • Elevated vaginal pH above 4.5

Associations

  • Bacterial vaginosis
  • Loss of protective lactobacilli
  • Polymicrobial overgrowth with anaerobes

Commonly confused with

  • Trichomonas vaginalis
  • Candida vulvovaginitis

Your notes

Original student-study summary. Sources checked: OpenStax Microbiology 2e, NCBI Bookshelf Medical Microbiology, and CDC topic pages where applicable; reviewed 2026-06. Educational only; no diagnosis, treatment, dosing, or specimen-handling guidance.

OpenStax: Microbiology 2e organism classification foundationssourceNCBI Bookshelf: Medical Microbiology organism chapterssourceCDC: CDC disease and public-health topic pagessource