PetriKey

Lab method

Quellung reaction

Antibody-induced capsular swelling

KVELL-oong

staincapsuleserotypingimmunologicpneumococcus

High-yield clue

Apparent capsular 'swelling' after adding specific antiserum is the classic Quellung clue for encapsulated pneumococcus.

Overview

A capsule-visualization concept in which type-specific anticapsular antibody binds a bacterium's polysaccharide capsule, making it appear swollen and sharply outlined under the microscope.

Classification

  • Immunologic capsule reaction
  • Serotyping concept
  • Light microscopy
  • Neufeld reaction synonym

Lab & identification clues

  • Capsule looks enlarged and refractile
  • Type-specific antisera define serotype
  • Gold-standard pneumococcal serotyping vocabulary
  • Also applies to other encapsulated bacteria

Associations

  • Streptococcus pneumoniae serotyping association
  • Also Klebsiella, Neisseria, and Haemophilus capsules
  • Capsule as virulence factor framing

Commonly confused with

  • India ink preparation
  • Latex agglutination

Your notes

Original concept summary for coursework. Sources checked: OpenStax Microbiology 2e and NCBI Bookshelf Medical Microbiology; reviewed 2026-06. Describes vocabulary and interpretation concepts only; not a lab protocol and not for handling specimens or identifying patient isolates.

OpenStax: Microbiology 2e staining, media, and biochemical-test foundationssourceNCBI Bookshelf: Medical Microbiology diagnostic concept foundationssource