PetriKey

Concept

Beta-lactamase

Enzyme that breaks the beta-lactam ring

BAY-tuh-LAK-tuh-mays

resistanceenzymeamrbeta-lactamhydrolysis

High-yield clue

Beta-lactamase inactivates penicillins by hydrolyzing (cleaving) the beta-lactam ring before the drug can act.

Overview

A bacterial enzyme that hydrolyzes the beta-lactam ring of penicillins and related drugs, inactivating them. It is the most common enzymatic mechanism of resistance to beta-lactam antibiotics.

Classification

  • Hydrolytic enzyme
  • Serine or metallo (zinc) active site
  • Ambler classes A-D
  • Chromosomal or plasmid-encoded

Lab & identification clues

  • Penicillinase vocabulary
  • Serine vs metallo-beta-lactamase concept
  • Beta-lactamase-inhibitor combination vocabulary

Associations

  • Penicillin and cephalosporin inactivation
  • Basis of ESBL and carbapenemase framing
  • Often plasmid-transmissible

Commonly confused with

  • Altered PBP (mecA) resistance
  • Porin loss

Your notes

Original microbiology concept summary. Sources checked: OpenStax Microbiology 2e, NCBI Bookshelf Medical Microbiology, and CDC/WHO topic pages where applicable; reviewed 2026-06. Educational only; no diagnosis, treatment selection, infection-control instructions, or specimen-handling guidance.

OpenStax: Microbiology 2e concept foundationssourceNCBI Bookshelf: Medical Microbiology general conceptssourceCDC: CDC public-health concept pagessource